EL GEN CRK33 ES REGULADO POR GIBERELINAS DURANTE EL DESARROLLO DEL FRUTO Arabiopsis thaliana (CRK33 GENE IS REGULATED BY GIBERELINES DURING THE DEVELOPMENT FRUIT IN Arabidopsis thaliana)
Resumen
Resumen
El fruto representa una parte importante en la dieta humana y animal. En Arabidopsis thaliana, el fruto es un excelente modelo de estudio por la forma de desarrollo y los tejidos que lo conforman. Sin embargo, aún es poca la información que se tiene sobre la regulación de su desarrollo, forma y tamaño. Existen un grupo de quinasas tipo receptor (RLK´s) que participan en diversas etapas del desarrollo de la planta, a pesar de que pocas se han caracterizado funcionalmente se sabe que participan en la transducción de señales. Sin embargo, no se ha reportado su función durante el desarrollo del fruto.
En el presente proyecto se aborda el estudio del gen CRK33, perteneciente a la familia de genes que codifican para proteínas quinasas de tipo receptor de RLK en plantas. En estudios recientes se ha reportado la participación e importancia de los reguladores de crecimiento vegetal o fitohormonas en el desarrollo del fruto, entre ellas las giberelinas. Hasta el momento, se ha demostrado que el gen CRK33se expresa en las hojas y frutos cuando son tratados con giberelinas exógenas, sugiriendo que éstas últimas lo regulan, no obstante, se están llevando a cabo análisis que confirmen lo antes mencionado. Por otro lado, se analizan los productos de las cruzas de la línea mutante salk-crk33 con líneas marcadoras del desarrollo del fruto a fin de encontrar cambios en el patrón de expresión.
Palabras Clave:Fruto, gen CRK33, giberelinas.
Abstract
The fruit represents an important part in the human and animal diet. In Arabidopsis thaliana, the fruit is an excellent study model because of the way of development and the tissues that make it up, however, there is little information about the regulation and induction of it. There is a group of receptor-type kinases (RLK´s) involved in various stages of plant development, although few have been functionally characterized as being involved in signal transduction. However, their function has not been reported during fruit development.
The present project addresses the study of the CRK33 gene, belonging to the family of genes coding for protein kinases of receptor type RLK in plants.
Recent studies have reported the participation and importance of plant growth regulators or phytohormones in the development of the fruit, including gibberellins.
So far, it has been demonstrated that the CRK33 gene is expressed in leaves and fruits when treated with exogenous gibberellins, suggesting that the latter regulates it, however, analyzes are being carried out confirming the aforementioned. On the other hand, the products of the crosses of the salk-crk33 mutant line with fruit development marker lines are analyzed in order to find changes in the expression pattern.
Keywords:Fruit, CRK33 gene, gibberellins.
Texto completo:
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